Cope-LLG Catalogue 2018

1293 13. Life Sciences Genomics/Thermocyclers GENERAL CATALOGUE EDITION 20 E & OE. Real-time PCR-system Prime Pro 48 The new Prime Pro 48 real time PCR system from Techne (UK) is a high specification, economically priced real-time thermocycler. The Prime Pro 48 system has a unique high thermal block uniformity (±0.1 °C). This uniformity as well as validated sample volumes of down to 5 µl enable an optimized reaction run time for 40 cycles down to only 15 minutes. The uniform temperature across the plate is achieved with a unique heating and cooling system that quickly enables a uniform temperature of ±0.1 °C in every well of the block within a fraction of a second of reaching each well temperature. This high uniformity eliminates the need for triplicates which reduces reagent running costs. Prime Pro 48 accommodates a unique and economical 48-well PCR plate, the size of which is only 1/8 the size of a standard 96-well plate. The well format mirrors a 384-well plate therefore allowing the use of a 16-channel micropipette. The small plate cuts reagent costs in half whilst still producing a strong fluorescent signal. Applications include e.g. absolute and relative quantification, Allelic Discrimination and High Resolution Melt. Features: - Validated volume per well: 5 to 20 µl - High temperature uniformity of ±0.1 °C across the whole block instantly after every temperature change - 40 cycles in 40 minutes before optimization, and down to only 15 minutes when optimized - Genotype in 4 minutes with over 99 % accuracy - Patented "Adaptive LED Control" minimizes optical artifacts and prevents premature detector saturation - Dual LED excitation (452 nm to 486 nm and 542 nm to 582 nm) - 4 emission filters (505 nm to 545 nm, 562 nm to 596 nm, 604 nm to 644 nm, 665 nm to 705 nm) - Data is always collected in all four filters for all wells - Distinguishes 5000 and 10000 template copies with 99 percent confidence Additional features: - 48-well block - MIQE compliant - 400 analyte specific qPCR reagent kits available - No calibration required - Calibrated dyes: SYBR ® , FAMTM, HEXTM, ROXTM, Cy ® 5. - Additional dyes within the filter wavelength range are supported with no additional calibration required - Unlimited software license - 1 year warranty Custom 48-well qPCR plates and corresponding seals have to be ordered separately. 1 Techne Specifications Temperature range: 30 to 100 °C Temperature stability: ±0.1 °C Temperature uniformity: ±0.1 °C within 1 sec Average ramp rate: 5.5 °C/sec Dimensions (W x D x H): 345 x 310 x 320 mm Weight: 13.6 kg Type PK Cat. No. Prime Pro 48 PCR plates Prime Pro 48 Plate seals Prime Pro 48 1 50 50 9.595 515 9.595 517 9.595 516 1 9.595 515