Cope-LLG Catalogue 2018

1400 14. Chromatography Vials/General information vials and caps GENERAL CATALOGUE EDITION 20 E & OE. Technical Information ʹ Vials In chromatography a broad variety of glass or plastic vials are used as sample containers for analysis usage. As they are mainly used within autosamplers or any other automatic instrument, strict obedience of all dimensions is crucial for a trouble-free run. Besides these physical properties the vials also have to fulfill requirements regarding inertness and cleanliness, as otherwise analysis results may be incorrect. LLG Labware consider the physical and chemical demands in their production process by various implementations: Almost all vials are made out of 1st hydrolytic class glass. First hydrolytic class glass is very hard and has a low expansion coefficient even at high temperature variations. It shows an excellent chemical resistance to acidic and neutral solutions, and even to alkaline solutions due to its relatively low Alkali content. Higher density of the glass surface offers a higher hydrolytic resistance. Clear Glass of 1st hydrolytic class is differentiated by 33 expansion (Type 1, Class A) and 51 expansion glass (Type 1, Class B), whereas amber is generally worldwide only available as 51 expansion glass. The indicated lower expansion coefficient of 33 implies that this harder clear glass has to be processed at higher temperatures. These amount to approx. 1,200°C for 33 expansion glass in comparison to only approx. 1,000°C for glass of 51 expansion. In the USA typically clear glass in 33 expansion and amber glass in 51 expansion is used, whereas in Europe solely 51 expansion glass is processed. From a quality point of view both types of glass are equally suitable for usage in chromatography, as they both are glasses of 1st hydrolytic class. Technical Information ʹ Seals Seals are the assembled combination of a cap and a septa. To carry out a correct analysis, it is important that besides the vial the seal is also inert and uncontaminated The closures sold by LLG Labware are automatically assembled and packed according to internally defined conditions of the supplier. Photocells check the side-orientation of the liner, so that it is ensured that the PTFE lamination is always directed towards the sample to build an inert barrier between sample and carrier material of the septa. A gauge control ensures that not more or less than one septa is installed. The final seals are automatically counted ʹ and not weighed ʹ by automates to guarantee quantity obedience. They are packed in tamper-proof evident zip-lock bags that allow easy identification of the content due to the transparent PE material. The zip-lock enables resealing of the bag to avoid any contamination of the closures during consumption. The batch number of the manufactured seal is printed on each PE-bag for traceability. Technical Information ʹ Septa The right choice of septa depends on the application. Almost all septa are laminated on one side with PTFE, which has a high chemical resistance and forms an inert barrier between sample and carrier material of the septa. The carrier materials have different physical and chemical properties, such as temperature resistance, resealability properties, cleanliness, hardness, thickness, etc. 7KH LQGLYLGXDO FRQGLWLRQV RI WKH FXVWRPHU¶V DSSOLFDWLRQ DLP DW WKH VSHFLILF FKDUDFWHULVWLFV RI WKH carrier material, e.g.: Multiple injection? good resealability properties necessary Natural Rubber/TEF Temperature? Thin, fragile needle? soft and thin septa required e.g. Silicone/PTFE Blunt, thick needle? Slitted/pre-cut liner as penetration aid (HPLC) e.g. 08 02 0039 Critical analysis? very clean liner required Silicone/ PTFE septa Low coring? Both sided PTFE laminated liners required PTFE/Silicone/PTFE PTFE/Butyl/PTFE -40°C up to 120°C Natural Rubber/ TEF; Butyl/ PTFE -40°C up to 110°C Red Rubber/ PTFE -60°C up to 200°C Silicone/ PTFE General information about vials and caps