Cope-LLG Catalogue 2018

1467 14. Chromatography Gas chromatography/GC reagents GENERAL CATALOGUE EDITION 20 E & OE. Reagents and methods for derivatisation For improved volatility, better thermal stability or low limit of detection in gas chromatography prerequisite: quantitative, rapid and reproducible formation of only on derivative halogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and fragmentation patterns in MS can be influenced by a specific derivatisation. Reagents for silylation - alkylation (methylation) - acylation are available. Derivatisation method development kit Which type of derivatisation is best suited for your sample (alkylation, silylation or acylation)? Test kit for determination of the optimum derivatisation. MACHEREY-NAGEL Content PK Cat. No. 2 x 1 ml each for TMSH, MSTFA, MBTFA 2 4.001 514 Acylation kit Which is the proper reagent for acylation? Test kit for determination of the optimum acylation. MACHEREY-NAGEL Content PK Cat. No. 2 x 1 ml each for MBTFA , TFAA, MBHFBA 1 6.207 118 Alkylation kit Which is the proper reagent for methylation? Test kit for determination of the optimum alkylation. MACHEREY-NAGEL Content PK Cat. No. 3x1 ml each for TMSH, DMF-DMA 3 4.001 513 Silylation kit Which is the proper reagent for silylation? Test kit for determination of the optimum silylation. MACHEREY-NAGEL Content PK Cat. No. 2 x 1 ml each for MSTFA, BSTFA, TSIM, MSHFBA 1 6.704 458 Silylation reagents - MSTFA N-methyl-N-trimethylsilyl-trifluoroacetamide m.w. 199.1, Bp 70 °C (75mm Hg), density d20°/4° = 1.11 MSTFA: R' = CF 3 , R" = CH 3 the most volatile trimethylsilyl amide available very strong TMS donor which does not cause any noticeable fouling of the FID burning chamber even after long-time measuring series The already good solution characteristics can be improved by addition of submolar quantities of protic solvents (e.g. TFA for extremely polar compounds such as hydrochlorides) or pyridine (e.g. for carbohydrates). recommended application: carboxylic acids, hydroxy and ketocarboxylic acids, amino acids, amines, alcohols, polyalcohols, sugars, mercaptans and similar compounds with active hydrogen atoms. Even amine hydrochlorides can be silylated directly. advantages: complete reaction with high reaction rates, even without a catalyst (1 to 2 % TMCS or TSIM) the by-product of the reaction (N-methyltrifluoroacetamide) features high volatility and short retention time. 1 MACHEREY-NAGEL Description Capacity PK Cat. No. ml MSTFA MSTFA MSTFA MSTFA MSTFA 1 10 10 50 100 20 1 5 6 6 7.055 892 6.704 091 6.085 475 6.227 450 4.001 493 Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture. The derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability and should be used soon. 1